human PPP1R12A shRNA silencing AAV

Name
AAV-h-PPP1R12A-shRNA

Cat No
shAAV-219607

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PPP1R12A.

shAAV-219607

AAV-h-PPP1R12A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPP1R12A (protein phosphatase 1 regulatory subunit 12A). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

M130; MBS; myosin binding subunit; Myosin phosphatase target subunit 1; myosin phosphatase, target subunit 1; myosin phosphatase-targeting subunit 1; MYPT1; protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A; protein phosphatase myosin-binding subunit

Description (eCommerce)

MYPT1 is the non-catalytic regulatory subunit of the myosin phosphatase (MP). MYPT1 is the smooth-muscle/platelet isoform. MYPT1 is a substrate of and is regulated by Rho-protein kinase. Phosphorylation of MYPT1 by Rho kinase inhibits MP activity.

Description (Vector)

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Gene ID

4659

Gene Name (eCommerce)

protein phosphatase 1 regulatory subunit 12A

Gene Name (Vector)

protein phosphatase 1 regulatory subunit 12A

Gene Symbol

PPP1R12A

HGNC ID

HGNC:7618

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

3093

ORF Size (bp)

3093 bp

Protein Name (eCommerce)

Protein phosphatase 1 regulatory subunit 12A

RefSeq ID

NM_002480

RefSeq Synonyms

NM_002480, NM_001244992, NM_001244990, NM_001143886, NM_001143885, BC111752,

Research Areas

Cancer,Cardiovascular,Cell Cycle,Cytoskeleton,Kinase/Phosphatase,PLK1 Signaling,Rho Signaling,Signal Transduction,TNF Signaling

Research Areas (Faceted)

cancer,cardiovascular,cell_biology,cell_cycle,genetics,signal_transduction

Species

human

Target Sentence

UniGene ID

Hs.49582

UniProt ID (eCommerce)

O14974

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.