mouse MDFI shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Mdfi.
shAAV-264404
AAV-m-MDFI-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Mdfi (MyoD family inhibitor). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
I-mf; I-mfa
Description (Vector)
Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).
Gene ID
17240
Gene Name (Vector)
MyoD family inhibitor
Gene Symbol
Mdfi
ORF Size (aa)
741
ORF Size (bp)
741 bp
RefSeq ID
NM_010783
RefSeq Synonyms
NM_010783, NM_001276391, NM_001276390, NM_001109973, BC085233, BC083058, BC010259, BC006018,
Species
mouse
UniGene ID
Mm.329100
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.