human UPF3A shRNA silencing AAV

Name
AAV-h-UPF3A-shRNA

Cat No
shAAV-227226

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human UPF3A.

shAAV-227226

AAV-h-UPF3A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human UPF3A (UPF3 regulator of nonsense transcripts homolog A (yeast)). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

HUPF3A; RENT3A; UPF3

Description (Vector)

UPF3A encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. UPF3A is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene.

Gene ID

65110

Gene Name (Vector)

UPF3 regulator of nonsense transcripts homolog A (yeast)

Gene Symbol

UPF3A

HGNC ID

HGNC:20332

ORF Size (aa)

1332

ORF Size (bp)

1332 bp

RefSeq ID

NM_023011

RefSeq Synonyms

NM_080687, NM_023011, BC023569,

Species

human

UniGene ID

Hs.533855

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.