human UPF3A shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human UPF3A.
shADV-227226
Ad-h-UPF3A-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human UPF3A (UPF3 regulator of nonsense transcripts homolog A (yeast)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
HUPF3A; RENT3A; UPF3
Description (Vector)
UPF3A encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. UPF3A is located on the long arm of chromosome 13. Two splice variants encoding different isoforms have been found for this gene.
Gene ID
65110
Gene Name (Vector)
UPF3 regulator of nonsense transcripts homolog A (yeast)
Gene Symbol
UPF3A
HGNC ID
HGNC:20332
ORF Size (aa)
1332
ORF Size (bp)
1332 bp
RefSeq ID
NM_023011
RefSeq Synonyms
NM_080687, NM_023011, BC023569,
Species
human
UniGene ID
Hs.533855
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.