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human DOHH shRNA silencing AAV

Name 
AAV-h-DOHH-shRNA
Cat No 
shAAV-207240
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human DOHH.

shAAV-207240
AAV-h-DOHH-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human DOHH (deoxyhypusine hydroxylase/monooxygenase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
hDOHH; HLRC1
Description (Vector)
DOHH encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.
Gene ID
83475
Gene Name (Vector)
deoxyhypusine hydroxylase/monooxygenase
Gene Symbol
DOHH
HGNC ID
HGNC:28662
ORF Size (aa)
909
ORF Size (bp)
909 bp
RefSeq ID
NM_031304
RefSeq Synonyms
NM_031304, NM_001145165, BC009863, BC002817,
Species
human
UniGene ID
Hs.609012

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.