human KRIT1 shRNA silencing AAV

Name
AAV-h-KRIT1-shRNA

Cat No
shAAV-213368

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human KRIT1.

shAAV-213368

AAV-h-KRIT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human KRIT1 (KRIT1, ankyrin repeat containing). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CAM; CCM1

Description (Vector)

KRIT1 encodes a protein containing four ankyrin repeats, a band 4.1/ezrin/radixin/moesin (FERM) domain, and multiple NPXY sequences. The encoded protein is localized in the nucleus and cytoplasm. It binds to integrin cytoplasmic domain-associated protein-1 alpha (ICAP1alpha), and plays a critical role in beta1-integrin-mediated cell proliferation. It associates with junction proteins and RAS-related protein 1A (Rap1A), which requires the encoded protein for maintaining the integrity of endothelial junctions. It is also a microtubule-associated protein and may play a role in microtubule targeting. Mutations in this gene result in cerebral cavernous malformations. Multiple alternatively spliced transcript variants have been found for this gene.

Gene ID

889

Gene Name (Vector)

KRIT1, ankyrin repeat containing

Gene Symbol

KRIT1

HGNC ID

HGNC:1573

ORF Size (aa)

2211

ORF Size (bp)

2211 bp

RefSeq ID

NM_004912

RefSeq Synonyms

NM_194456, NM_194455, NM_194454, NM_004912, NM_001013406, BC098442, BC094684,

Species

human

UniGene ID

Hs.531987

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.