mouse KRIT1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Krit1.
shAAV-263060
AAV-m-KRIT1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Krit1 (KRIT1, ankyrin repeat containing). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2010007K12Rik; A630036P20Rik; AA432855; AI450393; AI643869; BB155247; BB235701; Ccm1
Description (Vector)
Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity (By similarity). Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits EKR1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels.
Gene ID
79264
Gene Name (Vector)
KRIT1, ankyrin repeat containing
Gene Symbol
Krit1
ORF Size (aa)
2211
ORF Size (bp)
2211 bp
RefSeq ID
NM_030675
RefSeq Synonyms
NM_030675, NM_001170552, BC141042,
Species
mouse
UniGene ID
Mm.32368
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.