human KPNB1 shRNA silencing AAV

Name
AAV-h-KPNB1-shRNA

Cat No
shAAV-213350

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human KPNB1.

shAAV-213350

AAV-h-KPNB1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human KPNB1 (karyopherin subunit beta 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

IMB1; Impnb; importin 1; importin 90; importin beta-1 subunit; importin subunit beta-1; Importin-90; IPO1; IPOB; karyopherin (importin) beta 1; Karyopherin subunit beta-1; NTF97; nuclear factor p97; pore targeting complex 97 kDa subunit; PTAC97

Description (eCommerce)

KPNB1, also known as importin (karyopherin)-beta, plays an important role in the transport of macromolecules between the cytoplasm and nucleus. KPNB1 functions as a transport receptor that binds to importin-alpha-bound cargo and docks it to the cytoplasmic side of the nuclear pore complex (NPC) for translocation through the NPC.

Description (Vector)

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Gene ID

3837

Gene Name (eCommerce)

karyopherin subunit beta 1

Gene Name (Vector)

karyopherin subunit beta 1

Gene Symbol

KPNB1

HGNC ID

HGNC:6400

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

2631

ORF Size (bp)

2631 bp

Protein Name (eCommerce)

Importin subunit beta-1

RefSeq ID

NM_002265

RefSeq Synonyms

NM_002265, NM_001276453, BC036703, BC024045, BC003572,

Research Areas

Apoptosis,Cell Cycle,HGF Signaling,Host-Virus Interactions,Immunology,Metabolism/Metabolic Process,NFAT Signaling,Nuclear Import/Export,Ribonucleoprotein Transport,RNA Transport,Signal Transduction,TGF-beta Signaling,Transport

Research Areas (Faceted)

cell_biology,cell_cycle,genetics,immunology,infectious_disease,metabolism,signal_transduction,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.706168

UniProt ID (eCommerce)

Q14974

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.