human GRAP shRNA silencing AAV

Name
AAV-h-GRAP-shRNA

Cat No
shAAV-210481

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human GRAP.

shAAV-210481

AAV-h-GRAP-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GRAP (GRB2-related adaptor protein). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

GRAP encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic signaling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-terminal SH3 domain. In general, it couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.

Gene ID

10750

Gene Name (Vector)

GRB2-related adaptor protein

Gene Symbol

GRAP

HGNC ID

HGNC:4562

ORF Size (aa)

654

ORF Size (bp)

654 bp

RefSeq ID

NM_006613

RefSeq Synonyms

NM_006613, NM_001330148, BC063035, BC035856,

Species

human

UniGene ID

Hs.567416

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.