human FAM113A shRNA silencing AAV

Name
AAV-h-FAM113A-shRNA

Cat No
shAAV-208331

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human PCED1A.

shAAV-208331

AAV-h-FAM113A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PCED1A (PC-esterase domain containing 1A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

bA12M19.1; C20orf81; FAM113A

Description (Vector)

PCED1A is a member of the GDSL/SGNH superfamily. Members of this family are hydrolytic enzymes with esterase and lipase activity and broad substrate specificity. This protein belongs to the Pmr5-Cas1p-esterase subfamily in that it contains the catalytic triad comprised of serine, aspartate and histidine and lacks two conserved regions (glycine after strand S2 and GxND motif). A pseudogene of this gene has been identified on the long arm of chromosome 2. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

Gene ID

64773

Gene Name (Vector)

PC-esterase domain containing 1A

Gene Symbol

PCED1A

HGNC ID

HGNC:16212

ORF Size (aa)

1365

ORF Size (bp)

1365 bp

RefSeq ID

NM_022760

RefSeq Synonyms

NM_022760, NM_001271168, BC051816, BC037240, BC014247,

Species

human

UniGene ID

Hs.29341

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.