AAV with SST promoter driven Cre Inducible RFP

This AAV expresses DIO-RFP driven by an islet SST promoter.

The rat SST gene (also known as SS-14, SS-28, or Smst) is synthesized in the pancreatic islets, gastrointestinal tract, nervous system, and thyroid gland. The promoter (SSTp) is a 0.9Kb regulatory fragment from the rat SST gene.

In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5′ promoter and is flanked by oppositely oriented loxP and lox2272 sites. In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be “FLip-EXchanged” or FLEXed, leading to expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).