AAV with SST promoter driven fLuc

Name 
AAV-SST-fLuc-WPRE
Cat No 
VB5370
Availability
3 weeks
Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses fLuc driven by an islet SST promoter.

The rat SST gene (also known as SS-14, SS-28, or Smst) is synthesized in the pancreatic islets, gastrointestinal tract, nervous system, and thyroid gland. The promoter (SSTp) is a 0.9Kb regulatory fragment from the rat SST gene.

Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.

VB5370
AAV-SST-fLuc-WPRE

Ready-to-use AAV expressing fLuc driven by an islet SST promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
SST (islet)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.