AAV with CamKII(0.4) promoter driven Mito-roGFP1

Name 
AAV-CamKII(0.4)-Mito-roGFP1-WPRE
Cat No 
VB5204
Availability
3 weeks
Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses Mito-roGFP1 driven by a neuron CamKII(0.4) promoter.

This 0.4 Kb promoter is derived from murine α-Calcium/calmodulin-dependent kinase II (CaMKII), a gene with expression restricted to excitatory neurons in the neocortex and hippocampus. It was shown that the promoter activity of CaMKII(0.4) is almost identical to that of CaMKII(1.3) in cortical neurons, Compared to CaMKII(1.3), this short promoter allows cloning of longer gene of interest into the AAV construct .

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.

VB5204
AAV-CamKII(0.4)-Mito-roGFP1-WPRE

Ready-to-use AAV expressing Mito-roGFP1 driven by a neuron CamKII(0.4) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CamKII(0.4) (neuron)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.