AAV with hRPE(1.5) promoter driven mCitrine

Name
AAV-hRPE(1.5)-mCitrine-WPRE

Cat No
VB5001

Availability
3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses mCitrine driven by a retina hRPE(1.5) promoter.

The RPE65 gene encodes a protein of the retinal pigment epithelium (RPE) with a key role in the cycling of retinoids. This 1.5Kb promoter gives RPE-specific expression, but the expression level is not as high as that of the shorter, 0.8Kb, promoter. This is probably due to a negative regulatory element(s) present in the 1.5Kb promoter.

mCitrine, like Venus, is derived from YFP, with increased brightness and stability. mCitrine has an Excitation/Emission spectrum of 516nm/529nm which is almost identical to that of EYFP, Venus and Topaz.

VB5001

AAV-hRPE(1.5)-mCitrine-WPRE

Ready-to-use AAV expressing mCitrine driven by a retina hRPE(1.5) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

hRPE(1.5) (retina)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.