AAV with eSYN promoter driven mCherry

Name
AAV-eSYN-mCherry-WPRE

Cat No
VB4884

Availability
Immediate

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses mCherry driven by a neuron eSYN promoter.

The eSYN is a hybrid promoter consisting of a 0.4Kb CMV enhancer (E) and the 0.45Kb human Synapsin I promoter fragment (hSYN1). eSYN hybrid promoter gives about 2-4 fold higher expression than that of the parental hSYN1 promoter. However, the neuronal specificity of eSYN is slightly decreased, compared to that of the parental hSYN1 promoter.

mCherry was derived from DsRed through extensive mutagenesis. It has an Excitation/Emission spectrum of 590nm/610nm. mCherry’s emission spectrum is similar to the following fluorescent markers: mRuby (Em=605nM), JRed (Em=610nM), mRFP1 (Em=607nM), and HcRed1.

VB4884

AAV-eSYN-mCherry-WPRE

Ready-to-use AAV expressing mCherry driven by a neuron eSYN promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

eSYN (neuron)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.