AAV with EF1a promoter driven C1V1(E162T)-TS-mCitrine

Name 
AAV-EF1a-C1V1(E162T)-TS-mCitrine
Cat No 
VB4560
Availability
3 weeks
Categories
Optogenetics, AAV, Over-Expression

This AAV expresses C1V1(E162T)-TS-mCitrine driven by an ubiquitous EF1a promoter.

The 1.2 Kb EF1α promoter is a commonly used, constitutive promoter derived from human elongation factor-1 alpha (EF1α). It is often used in conditions where the other constitutive promoters, such as CMV, have diminished activity or have been silenced.

C1V1 is a chimeric channelrhodopsin made to boost membrane expression higher than VChR1 but with a different light spectrum. It is composed of ChR1 and VChR1 fragments, and implements fast, potent optical excitation at red-shifted wavelengths. C1V1(E162T) mutant has an off-kinetic is half of C1V1.

The trafficking signal (KSRITSEGEYIPLDQIDINV) is also from the inward rectifier potassium channel Kir2.1; it serves to dramatically reduce intracellular accumulation and improve membrane targeting, leading to a profound increase of photocurrents. This is the basis for many third-generation optogenetics tools.

VB4560
AAV-EF1a-C1V1(E162T)-TS-mCitrine

Ready-to-use AAV expressing C1V1(E162T)-TS-mCitrine driven by an ubiquitous EF1a promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
EF1a (ubiquitous)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.