AAV with MBP promoter driven hChR2(H134R)-ECFP

Name 
AAV-MBP-hChR2(H134R)-ECFP
Cat No 
VB4176
Availability
3 weeks
Categories
Optogenetics, AAV, Over-Expression

This AAV expresses hChR2(H134R)-ECFP driven by an olgiodendricyte MBP promoter.

The myelin basic proteins (MBPs) are a family of polypeptides that are predominantly expressed in the nervous system, where they play a major role in myelination. This 1.3 Kb mouse MBP promoter drives reporter gene expression mainly in oligodendrocytes.

Channelrhodopsins are light-gated ion channels that exist naturally in microalgae. hChR2 is a humanized version of ChR2 for mammalian expression. It is maximally excited by 470 nm light. The wild-type, as well as a few mutations, provide the fastest excitation of the channelrhodopsins offered, and are widely used in optogenetics techniques in neuroscience. Due to delayed channel closure, hChR2(H134R) is a gain-of-function mutation that produces larger photocurrents than wild-type hChR2, but slows down channel kinetics.

VB4176
AAV-MBP-hChR2(H134R)-ECFP

Ready-to-use AAV expressing hChR2(H134R)-ECFP driven by an olgiodendricyte MBP promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
MBP (olgiodendricyte)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.