AAV with CamKII(0.4) promoter driven hChR2(E123T/H134R)-TdTomato

Name
AAV-CamKII(0.4)-hChR2(E123T/H134R)-TdTomato

Cat No
VB3518

Availability
3 weeks

Categories
Optogenetics, AAV, Over-Expression

This AAV expresses hChR2(E123T/H134R)-TdTomato driven by a neuron CamKII(0.4) promoter.

This 0.4 Kb promoter is derived from murine α-Calcium/calmodulin-dependent kinase II (CaMKII), a gene with expression restricted to excitatory neurons in the neocortex and hippocampus. It was shown that the promoter activity of CaMKII(0.4) is almost identical to that of CaMKII(1.3) in cortical neurons, Compared to CaMKII(1.3), this short promoter allows cloning of longer gene of interest into the AAV construct .

hChR2(E123T/H134R), also known as ChETA, is a channelrhodopsin-2 variant with faster deactivation kinetics.

VB3518

AAV-CamKII(0.4)-hChR2(E123T/H134R)-TdTomato

Ready-to-use AAV expressing hChR2(E123T/H134R)-TdTomato driven by a neuron CamKII(0.4) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

CamKII(0.4) (neuron)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.