AAV with CMV promoter driven Cre Inducible hChR2(E123T/H134R)-TdTomato

Name 
AAV-cDNA6-DIO-hChR2(E123T/H134R)-TdTomato
Cat No 
VB3253
Availability
3 weeks
Categories
Optogenetics, AAV, Over-Expression

This AAV expresses DIO-hChR2(E123T/H134R)-TdTomato driven by an ubiquitous CMV promoter.

This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).

hChR2(E123T/H134R), also known as ChETA, is a channelrhodopsin-2 variant with faster deactivation kinetics.

In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5′ promoter and is flanked by oppositely oriented loxP and lox2272 sites. In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be “FLip-EXchanged” or FLEXed, leading to expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).

VB3253
AAV-cDNA6-DIO-hChR2(E123T/H134R)-TdTomato

Ready-to-use AAV expressing DIO-hChR2(E123T/H134R)-TdTomato driven by an ubiquitous CMV promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (ubiquitous)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.