AAV with CMV promoter driven hChR2(T159C)-TdTomato
This AAV expresses hChR2(T159C)-TdTomato driven by an ubiquitous CMV promoter.
This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).
hChR2 is a humanized version of ChR2 for mammalian expression. The wild-type, as well as a few mutations, provide the fastest excitation of the channelrhodopsins offered, and are widely used in optogenetics techniques in neuroscience. hChR2(T159C) shows slightly higher photocurrents than the hChR2(H134R), another commonly used optogenetics tool.
VB3238
AAV-cDNA6-hChR2(T159C)-TdTomato
Ready-to-use AAV expressing hChR2(T159C)-TdTomato driven by an ubiquitous CMV promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.