AAV with CMV promoter driven eNpHR3.0-TdTomato

Name 
AAV-cDNA6-eNpHR3.0-TdTomato
Cat No 
VB3089
Availability
Immediate
Categories
Optogenetics, AAV, Over-Expression

This AAV expresses eNpHR3.0-TdTomato driven by an ubiquitous CMV promoter.

This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).

NpHR is a halorhodopsin or a light-sensitive chloride channel that provides silencing of neuronal activity by hyperpolarization. It offers fast inhibition, and its peak activation falls at 589 nm.

The trafficking signal (KSRITSEGEYIPLDQIDINV) is also from the inward rectifier potassium channel Kir2.1; it serves to dramatically reduce intracellular accumulation and improve membrane targeting, leading to a profound increase of photocurrents. This is the basis for many third-generation optogenetics tools.

This ER export motif (FCYENEV) is from the vertebrate inward rectifier potassium channel Kir2.1. The motif prevents aggregate formation, decreases intracellular accumulations, and enhances tolerability at high expression levels. This is the strategy for developing many second-generation optogenetics tools.

VB3089
AAV-cDNA6-eNpHR3.0-TdTomato

Ready-to-use AAV expressing eNpHR3.0-TdTomato driven by an ubiquitous CMV promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (ubiquitous)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume (µL)
200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.