AAV with GFAP(0.7) promoter driven VChR1-Venus

Name 
AAV-GFAP(0.7)-VChR1-Venus
Cat No 
VB2511
Availability
2-3 weeks
Categories
Optogenetics, AAV, Over-Expression

This AAV expresses VChR1-Venus driven by an astrocyte GFAP(0.7) promoter.

This 0.7 Kb novel GFAP promoter was constructed by ligating several key regulatory elements of the 2.2Kb human GFAP promoter. It drives essentially the same expression pattern as the parental 2.2 Kb GFAP promoter but with two-fold greater activity.

Volvox channelrhodopsin-1 (VChR1) is from the multicellular green alga Volvox carteri. VChR1 has a similar functionality as hChR2 but with a different excitation spectrum towards green light, and shows action potential firing with 589 nm light.

VB2511
AAV-GFAP(0.7)-VChR1-Venus

Ready-to-use AAV expressing VChR1-Venus driven by an astrocyte GFAP(0.7) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
GFAP(0.7) (astrocyte)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume
100 µl, 250 µl, 500 µl, and 1ml

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.