AAV with ALB(1.4) promoter driven Mito-roGFP1
This AAV expresses Mito-roGFP1 driven by a liver ALB(1.4) promoter.
The ALB(1.4) is a shorter version of the ALB(1.7) promoter with a total length of 1.4 Kb. In mouse liver cells, the transgene expression from this ALB(1.4) synthetic promoter is about 3 times lower than that of ALB(1.7) promoter but 10-100 fold greater than that of the CMV promoter 10-20 weeks after plasmid injection into mouse liver.
The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.
Ready-to-use AAV expressing Mito-roGFP1 driven by a liver ALB(1.4) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.