AAV with ALB(1.4) promoter driven Mito-roGFP1

Name
AAV-ALB(1.4)-Mito-roGFP1

Cat No
VB1656

Availability
3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses Mito-roGFP1 driven by a liver ALB(1.4) promoter.

The ALB(1.4) is a shorter version of the ALB(1.7) promoter with a total length of 1.4 Kb. In mouse liver cells, the transgene expression from this ALB(1.4) synthetic promoter is about 3 times lower than that of ALB(1.7) promoter but 10-100 fold greater than that of the CMV promoter 10-20 weeks after plasmid injection into mouse liver.

The mitochondrial targeting sequence of human pyruvate dehydrogenase alpha 1 (PDHA1) is fused in-frame with the redox-sensitive green fluorescent protein (roGFP1). The Mito-roGFP1 is a biosensor for investigating the mitochondrial reduction-oxidation potential.

VB1656

AAV-ALB(1.4)-Mito-roGFP1

Ready-to-use AAV expressing Mito-roGFP1 driven by a liver ALB(1.4) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

ALB(1.4) (liver)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.