AAV with ALB(1.9) promoter driven mCherry

This AAV expresses mCherry driven by a liver ALB(1.9) promoter.

The ALB(1.9) is a synthetic promoter of ~1.9 Kb. It is based on the human Albumin promoter as well as several other regulatory elements. This synthetic promoter gives an extremely high level of long-term, liver-specific transgene expression that is 100-100 fold greater than that of the CMV promoter. For short-term (1-2 weeks post-injection into mouse liver), ALB(1.9) gives comparable expression relative to that of the CMV promoter. However, the expression level of the CMV promoter decreases 100-1000 fold from its peak level within 10-20 weeks of DNA administration whereas the ALB(1.9) promoter sustains its initial high level of expression for over 1 year. Compared to the regular ALB promoter, which is also commonly used for liver-specific transgene expression, the expression level from ALB(1.9) is about 10-100 fold greater.

mCherry was derived from DsRed through extensive mutagenesis. It has an Excitation/Emission spectrum of 590nm/610nm. mCherry’s emission spectrum is similar to the following fluorescent markers: mRuby (Em=605nM), JRed (Em=610nM), mRFP1 (Em=607nM), and HcRed1.