AAV with CMV promoter driven Cre Inducible EGFP
This AAV expresses DIO-EGFP driven by an ubiquitous CMV promoter.
This roughly 0.6 Kb promoter is the commonly used human cytomegalovirus (CMV) immediate-early promoter/enhancer (CMV/IE).
In the DIO scenario, the transgene of interest is inserted in reverse orientation relative to the 5′ promoter and is flanked by oppositely oriented loxP and lox2272 sites. In the absence of Cre expression, the transgene will not be produced. In the presence of Cre expression, the transgene will be “FLip-EXchanged” or FLEXed, leading to expression of the transgene. This is due to a permanent Cre-mediated recombination/inversion of the flanked transgene. This arrangement is called DIO (double-floxed inverse ORF), Cre-ON, Flex-rev (reverse), Flex-ON/FlexON, or DIO-AAV/AAV-DIO (double-floxed inverse ORF in AAV).
Ready-to-use AAV expressing DIO-EGFP driven by an ubiquitous CMV promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.
Related Citations
- MC4R Signaling in Dorsal Raphe Nucleus Controls Feeding, Anxiety, and Depression. G Bruschetta, etc, (2020), Cell Reports
- Overexpression of Melanocortin 2 Receptor Accessory Protein 2 (MRAP2) in adult paraventricular MC4R neurons regulates energy intake and expenditure. G Bruschetta, etc, (2018), Molecular Metabolism