AAV with hRPE(0.8) promoter driven ECFP

Name
AAV-hRPE(0.8)-ECFP

Cat No
VB1204

Availability
3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses ECFP driven by a retina hRPE(0.8) promoter.

The RPE65 gene encodes a protein of the retinal pigment epithelium (RPE) with a key role in the cycling of retinoids. It was shown that a RPE promoter of about 0.8 Kb was sufficient to direct high RPE-specific expression in transgenic mice. The 1.5Kb version of the promoter hRPE(1.5), also gives RPE-specific expression, but the expression level is not as high as the 0.8Kb promoter. This is probably due to a negative regulatory element(s) present in the 1.5Kb promoter.

Enhanced Cyan fluorescence protein(ECFP) is a cyan variant from the Aequorea GFP, with a Excitation/Emission spectrum around 434nm/477nm. Other versions of fluorescent proteins with similar excitation and emission spectrums include mcerulean3, mTurquoise, and SCFP.

VB1204

AAV-hRPE(0.8)-ECFP

Ready-to-use AAV expressing ECFP driven by a retina hRPE(0.8) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

hRPE(0.8) (retina)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.