AAV with GFAP(2.2) promoter driven TdTomato

Name 
AAV-GFAP(2.2)-TdTomato
Cat No 
VB1177
Availability
2-3 weeks
Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses TdTomato driven by an astrocyte GFAP(2.2) promoter.

The GFAP(2.2) promoter is based on human glial fibrillary acidic protein gene (GFAP), which is the major intermediate filament protein in astrocytes. It has been shown that approximately 2 kb of 5′-flanking DNA of the human GFAP gene is sufficient to direct transgene expression to astrocytes and confer developmental/injury-induced regulation.

dTomato belongs to a family of fluorescent proteins called mFruit. Two dTomato units were joined head-to-tail through a linker, yielding a tandem dimer fluorescent protein, tdTomato. The tdTomato derivative is the brightest fluorescent protein yet reported, featuring an orange-red emission maximum at 581nm and is one of the most photo-stable fluorescent proteins. Other fluorescent proteins with similar emission spectra include DsRed, DsRed2, TurboRFP, TagRFP, and TagRFP-T.

VB1177
AAV-GFAP(2.2)-TdTomato

Ready-to-use AAV expressing TdTomato driven by an astrocyte GFAP(2.2) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
GFAP(2.2) (astrocyte)
Storage Buffer
PBS/5% Glycerol
Titer
1x10^13 GC/ml
Viral Backbone
Recombinant AAV
Volume
100 µl, 250 µl, 500 µl, and 1ml

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.