AAV with GFAP(0.7) promoter driven EYFP

Name
AAV-GFAP(0.7)-EYFP

Cat No
VB1157

Availability
3 weeks

Categories
AAV, Control/Reporter, Over-Expression

This AAV expresses EYFP driven by an astrocyte GFAP(0.7) promoter.

This 0.7 Kb novel GFAP promoter was constructed by ligating several key regulatory elements of the 2.2Kb human GFAP promoter. It drives essentially the same expression pattern as the parental 2.2 Kb GFAP promoter but with two-fold greater activity.

YFP is the longest wavelength-emitting variants of Aequorea GFP. The enhanced version, EYFP, has become one of the brightest and most widely utilized of the fluorescent proteins with an Excitation/Emission spectrum centered around 514nm/527nm. Other fluorescent proteins with similar emission spectrums include Topaz, Venus, Citrine and mCitrine.

VB1157

AAV-GFAP(0.7)-EYFP

Ready-to-use AAV expressing EYFP driven by an astrocyte GFAP(0.7) promoter. Available in AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAV-DJ and other serotypes.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

GFAP(0.7) (astrocyte)

Storage Buffer

PBS/5% Glycerol

Titer

1x10^13 GC/ml

Viral Backbone

Recombinant AAV

Volume (µL)

200

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.