rat MED22 shRNA silencing Adenovirus

Name
Ad-r-MED22-shRNA

Cat No
shADV-295103

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Med22.

shADV-295103

Ad-r-MED22-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Med22 (mediator complex subunit 22). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

RGD1564893; Surf5

Description (Vector)

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene ID

499762

Gene Name (Vector)

mediator complex subunit 22

Gene Symbol

Med22

ORF Size (aa)

603

ORF Size (bp)

603 bp

RefSeq ID

NM_001077679

RefSeq Synonyms

NM_001077679, BC127519,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.