rat OPA1 shRNA silencing Adenovirus

Name
Ad-r-OPA1-shRNA

Cat No
shADV-294796

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Opa1.

shADV-294796

Ad-r-OPA1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Opa1 (OPA1, mitochondrial dynamin like GTPase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space|Function: Dynamin-like 120 kDa protein, form S1: Inactive form produced by cleavage at S1 position by OMA1 following stress conditions that induce loss of mitochondrial membrane potential, leading to negative regulation of mitochondrial fusion.

Gene ID

171116

Gene Name (Vector)

OPA1, mitochondrial dynamin like GTPase

Gene Symbol

Opa1

ORF Size (aa)

2883

ORF Size (bp)

2883 bp

RefSeq ID

NM_133585

RefSeq Synonyms

NM_133585, BC111071,

Species

rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.