rat TSEN2 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Rat Tsen2.
shADV-292593
Ad-r-TSEN2-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Rat Tsen2 (tRNA splicing endonuclease subunit 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
RGD1309946
Description (Vector)
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
Gene ID
312649
Gene Name (Vector)
tRNA splicing endonuclease subunit 2
Gene Symbol
Tsen2
ORF Size (aa)
1392
ORF Size (bp)
1392 bp
RefSeq ID
NM_001014057
RefSeq Synonyms
NM_001014057, BC087631,
Species
rat
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.