rat ATG3 shRNA silencing Adenovirus

Name 
Ad-r-ATG3-shRNA
Cat No 
shADV-291934
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Rat Atg3.

shADV-291934
Ad-r-ATG3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Rat Atg3 (autophagy related 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
97
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Apg3l; PIG-1; Pig1
Description (Vector)
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Gene ID
171415
Gene Name (Vector)
autophagy related 3
Gene Symbol
Atg3
ORF Size (aa)
945
ORF Size (bp)
945 bp
RefSeq ID
NM_134394
RefSeq Synonyms
NM_134394, BC078743,
Species
rat

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.