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mouse ZC3HC1 shRNA silencing Adenovirus

Name 
Ad-m-ZC3HC1-shRNA
Cat No 
shADV-281217
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Zc3hc1.

shADV-281217
Ad-m-ZC3HC1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Zc3hc1 (zinc finger, C3HC type 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1110054L24Rik; AU018540; AU019789; HSPC216; Nipa
Description (Vector)
Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events.
Gene ID
232679
Gene Name (Vector)
zinc finger, C3HC type 1
Gene Symbol
Zc3hc1
ORF Size (aa)
1506
ORF Size (bp)
1506 bp
RefSeq ID
NM_172735
RefSeq Synonyms
NM_172735, NM_001311086, BC050141,
Species
mouse
UniGene ID
Mm.29780

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.