mouse TNPO1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Tnpo1.
shADV-281172
Ad-m-TNPO1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Tnpo1 (transportin 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AU021749; D13Ertd688e; IPO2; Kpnb2; MIP; MIP1; TRN
Description (Vector)
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev.
Gene ID
238799
Gene Name (Vector)
transportin 1
Gene Symbol
Tnpo1
ORF Size (aa)
2673
ORF Size (bp)
2673 bp
RefSeq ID
NM_178716
RefSeq Synonyms
NM_178716, NM_001048267, BC055372,
Species
mouse
UniGene ID
Mm.173286
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.