mouse TRPM8 shRNA silencing Adenovirus

Name 
Ad-m-TRPM8-shRNA
Cat No 
shADV-275100
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Trpm8.

shADV-275100
Ad-m-TRPM8-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Trpm8 (transient receptor potential cation channel, subfamily M, member 8). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CMR1; LTrpC-6; LTRPC6; Trp-p8; TRPP8
Description (Vector)
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Gene ID
171382
Gene Name (Vector)
transient receptor potential cation channel, subfamily M, member 8
Gene Symbol
Trpm8
ORF Size (aa)
3315
ORF Size (bp)
3315 bp
RefSeq ID
NM_134252
RefSeq Synonyms
NM_134252, BC117934,
Species
mouse
UniGene ID
Mm.218753

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.