mouse SMAD4 shRNA silencing Adenovirus

Name 
Ad-m-SMAD4-shRNA
Cat No 
shADV-272602
Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Mouse Smad4.

shADV-272602
Ad-m-SMAD4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Smad4 (SMAD family member 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AW743858; D18Wsu70e; DPC4; Madh4
Description (Vector)
Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Gene ID
17128
Gene Name (Vector)
SMAD family member 4
Gene Symbol
Smad4
ORF Size (aa)
1656
ORF Size (bp)
1656 bp
RefSeq ID
NM_008540
RefSeq Synonyms
NM_008540, BC046584,
Species
mouse
UniGene ID
Mm.100399

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.