mouse SEMA4F shRNA silencing Adenovirus

Name 
Ad-m-SEMA4F-shRNA
Cat No 
shADV-271592
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse SEMA4F.

shADV-271592
Ad-m-SEMA4F-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse SEMA4F (sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Sema W
Description (Vector)
Has growth cone collapse activity against retinal ganglion-cell axons (By similarity).
Gene ID
20355
Gene Name (Vector)
sema domain, immunoglobulin domain (Ig), TM domain, and short cytoplasmic domain
Gene Symbol
SEMA4F
ORF Size (aa)
2334
ORF Size (bp)
2334 bp
RefSeq ID
NM_011350
RefSeq Synonyms
NM_011350, NM_001308374, NM_001113481, BC139232, BC094567,
Species
mouse
UniGene ID
Mm.270543

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.