mouse RTCD1 shRNA silencing Adenovirus

Name
Ad-m-RTCD1-shRNA

Cat No
shADV-271203

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Rtca.

shADV-271203

Ad-m-RTCD1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Rtca (RNA 3′-terminal phosphate cyclase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

2310009A18Rik; AI450277; RPC; Rtcd1

Description (Vector)

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.

Gene ID

66368

Gene Name (Vector)

RNA 3'-terminal phosphate cyclase

Gene Symbol

Rtca

ORF Size (aa)

1101

ORF Size (bp)

1101 bp

RefSeq ID

NM_025517

RefSeq Synonyms

NM_025517, BC090403, BC016519,

Species

mouse

UniGene ID

Mm.251228

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.