mouse PNPT1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Pnpt1.
shADV-268981
Ad-m-PNPT1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Pnpt1 (polyribonucleotide nucleotidyltransferase 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
1200003F12Rik; Old35; PNPase; Pnptl1
Description (Vector)
RNA-binding protein implicated in numerous RNA metabolic processes. Hydrolyzes single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.
Gene ID
71701
Gene Name (Vector)
polyribonucleotide nucleotidyltransferase 1
Gene Symbol
Pnpt1
ORF Size (aa)
2352
ORF Size (bp)
2352 bp
RefSeq ID
NM_027869
RefSeq Synonyms
NM_027869, BC055826,
Species
mouse
UniGene ID
Mm.211131
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.