mouse PHAX shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Phax.
shADV-268509
Ad-m-PHAX-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Phax (phosphorylated adaptor for RNA export). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2810055C14Rik; 4933427L19Rik; AU018701; AU018854; D18Ertd65e; p55; Rnuxa
Description (Vector)
A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.
Gene ID
56698
Gene Name (Vector)
phosphorylated adaptor for RNA export
Gene Symbol
Phax
ORF Size (aa)
1158
ORF Size (bp)
1158 bp
RefSeq ID
NM_019996
RefSeq Synonyms
NM_019996, NM_001162989, BC061110, BC049590,
Species
mouse
UniGene ID
Mm.214569
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.