mouse NR5A1 shRNA silencing Adenovirus

Name 
Ad-m-NR5A1-shRNA
Cat No 
shADV-266060
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Nr5a1.

shADV-266060
Ad-m-NR5A1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Nr5a1 (nuclear receptor subfamily 5, group A, member 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Ad4BP; ELP; ELP-3; Ftz-F1; Ftzf1; SF-1; SF1; STF-1
Description (Vector)
Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Gene ID
26423
Gene Name (Vector)
nuclear receptor subfamily 5, group A, member 1
Gene Symbol
Nr5a1
ORF Size (aa)
1389
ORF Size (bp)
1389 bp
RefSeq ID
NM_139051
RefSeq Synonyms
NM_139051, NM_001316687, BC110477, BC110476,
Species
mouse
UniGene ID
Mm.31387

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.