mouse NGLY1 shRNA silencing Adenovirus

Name 
Ad-m-NGLY1-shRNA
Cat No 
shADV-265748
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Ngly1.

shADV-265748
Ad-m-NGLY1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Ngly1 (N-glycanase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1110002C09Rik; Png1; PNGase
Description (Vector)
Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulun that are exported in the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.
Gene ID
59007
Gene Name (Vector)
N-glycanase 1
Gene Symbol
Ngly1
ORF Size (aa)
1956
ORF Size (bp)
1956 bp
RefSeq ID
NM_021504
RefSeq Synonyms
NM_021504, BC028961,
Species
mouse
UniGene ID
Mm.258381

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.