mouse MICAL3 shRNA silencing Adenovirus

Name 
Ad-m-MICAL3-shRNA
Cat No 
shADV-264627
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Mical3.

shADV-264627
Ad-m-MICAL3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Mical3 (microtubule associated monooxygenase, calponin and LIM domain containing 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
C130040D16Rik; Mical-3; mKIAA1364
Description (Vector)
Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (By similarity). Involved in exocytic vesicles tethering and fusion: the monooxygenase activity is required for this process.
Gene ID
194401
Gene Name (Vector)
microtubule associated monooxygenase, calponin and LIM domain containing 3
Gene Symbol
Mical3
ORF Size (aa)
2595
ORF Size (bp)
2595 bp
RefSeq ID
NM_153396
RefSeq Synonyms
NM_153396, NM_001270475, BC138257,
Species
mouse
UniGene ID
Mm.122399

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.