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mouse GLI3 shRNA silencing Adenovirus

Name 
Ad-m-GLI3-shRNA
Cat No 
shADV-260175
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Gli3.

shADV-260175
Ad-m-GLI3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Gli3 (GLI-Kruppel family member GLI3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
add; AI854843; AU023367; Bph; GLI3-190; GLI3FL; Pdn; Xt
Description (Vector)
Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
Gene ID
14634
Gene Name (Vector)
GLI-Kruppel family member GLI3
Gene Symbol
Gli3
ORF Size (aa)
4752
ORF Size (bp)
4752 bp
RefSeq ID
NM_008130
RefSeq Synonyms
NM_008130, BC145445, BC141135,
Species
mouse
UniGene ID
Mm.5098

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.