Promotional banner reading 'FALL SAVINGS' with expiry date August 31 on an orange background; features a group of people with orange stacked blocks on the right and discounts for services and catalog products.

human GLI3 shRNA silencing AAV

Name 
AAV-h-GLI3-shRNA
Cat No 
shAAV-209967
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human GLI3.

shAAV-209967
AAV-h-GLI3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human GLI3 (GLI family zinc finger 3). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ACLS; GCPS; GLI3 form of 190 kDa; GLI3 full-length protein; GLI3-190; GLI3FL; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3; oncogene GLI3; PAPA; PAP-A; PAPA1; PAPB; PHS; PPDIV; transcriptional activator GLI3; zinc finger protein GLI3
Description (eCommerce)
GLI3 (GLI family zinc finger 3) plays a role in limb development and has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway. After phosphorylation and nuclear translocation, the full-length form of GLI3 (GLI3FL), acts as an activator (GLI3A) while the C-terminally truncated form (GLI3R) acts as a repressor [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P10071].
Description (Vector)
GLI3 encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
Gene ID
2737
Gene Name (eCommerce)
GLI family zinc finger 3
Gene Name (Vector)
GLI family zinc finger 3
Gene Symbol
GLI3
HGNC ID
HGNC:4319
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4743
ORF Size (bp)
4743 bp
Protein Name (eCommerce)
Transcriptional activator GLI3
RefSeq ID
NM_000168
RefSeq Synonyms
NM_000168, BC117168, BC113616,
Research Areas
Apoptosis,Axon Guidance,Cancer,Cardiac Development,Cardiology,Cardiovascular,Hedgehog Signaling,Immunology,Neurobiology,Neurodevelopment,Signal Transduction,Transcription Factor/Regulator,Wnt Signaling,Zinc-finger
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,immunology,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
GLI3 (GLI family zinc finger 3) plays a role in limb development and has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway. After phosphorylation and nuclear translocation, the full-length form of GLI3 (GLI3FL), acts as an activator (GLI3A) while the C-terminally truncated form (GLI3R) acts as a repressor [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P10071].
UniGene ID
Hs.21509
UniProt ID (eCommerce)
P10071

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.