mouse GFI1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Gfi1.
shADV-260043
Ad-m-GFI1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Gfi1 (growth factor independent 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AW495828; Gfi-1; Pal-1; Pal1
Description (Vector)
Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells.
Gene ID
14581
Gene Name (Vector)
growth factor independent 1
Gene Symbol
Gfi1
ORF Size (aa)
1272
ORF Size (bp)
1272 bp
RefSeq ID
NM_010278
RefSeq Synonyms
NM_010278, NM_001267621, BC160313,
Species
mouse
UniGene ID
Mm.453139
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.