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mouse FREQ shRNA silencing Adenovirus

Name 
Ad-m-FREQ-shRNA
Cat No 
shADV-259627
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Ncs1.

shADV-259627
Ad-m-FREQ-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Ncs1 (neuronal calcium sensor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
9430075O15Rik; A730032G13Rik; AI836659; Freq; Mfreq; NCS-1
Description (Vector)
Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).
Gene ID
14299
Gene Name (Vector)
neuronal calcium sensor 1
Gene Symbol
Ncs1
ORF Size (aa)
573
RefSeq ID
NM_019681
RefSeq Synonyms
NM_019681, BC059825,
Species
mouse
UniGene ID
Mm.249318

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.