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human FREQ shRNA silencing AAV

Name 
AAV-h-FREQ-shRNA
Cat No 
shAAV-209308
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human NCS1.

shAAV-209308
AAV-h-FREQ-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human NCS1 (neuronal calcium sensor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
FLUP; FREQ
Description (Vector)
NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
23413
Gene Name (Vector)
neuronal calcium sensor 1
Gene Symbol
NCS1
HGNC ID
HGNC:3953
ORF Size (aa)
573
RefSeq ID
NM_014286
RefSeq Synonyms
NM_014286, NM_001128826, BC004856,
Species
human
UniGene ID
Hs.642946

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.