mouse LOXL2 shRNA silencing Adenovirus

Name 
Ad-m-LOXL2-shRNA
Cat No 
shADV-258497
Availability
Immediate

This is an Adenovirus expressing shRNA for silencing of Mouse Loxl2.

shADV-258497
Ad-m-LOXL2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Loxl2 (lysyl oxidase-like 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
91
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1110004B06Rik; 4930526G11Rik; 9430067E15Rik
Description (Vector)
Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation.
Gene ID
94352
Gene Name (Vector)
lysyl oxidase-like 2
Gene Symbol
Loxl2
ORF Size (aa)
1182
ORF Size (bp)
1182 bp
RefSeq ID
NM_033325
RefSeq Synonyms
NM_033325, BC086801,
Species
mouse
UniGene ID
Mm.116714

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.