mouse EIF3G shRNA silencing Adenovirus

Name 
Ad-m-EIF3G-shRNA
Cat No 
shADV-258211
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Eif3g.

shADV-258211
Ad-m-EIF3G-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Eif3g (eukaryotic translation initiation factor 3, subunit G). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
44kDa; D0Jmb4; eIF3-p44; Eif3s4; p44; TU-189B2
Description (Vector)
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
Gene ID
53356
Gene Name (Vector)
eukaryotic translation initiation factor 3, subunit G
Gene Symbol
Eif3g
ORF Size (aa)
963
ORF Size (bp)
963 bp
RefSeq ID
NM_016876
RefSeq Synonyms
NM_016876, BC008511,
Species
mouse
UniGene ID
Mm.427309

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.